Research Assistant I Lab

Location: Cambridge, MA, us Department: Sciences - Stem Cell and Regenerative Biology Employment Type: {'id': 'permanent', 'label': 'Full-time'}

Company Description

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Why join the Harvard Faculty of Arts and Sciences?

The Faculty of Arts and Sciences (FAS) is the historic heart of Harvard University. It is the home of Harvard’s undergraduate program (Harvard College, founded in 1636) as well as all of Harvard’s Ph.D. programs (the Harvard Kenneth C. Griffin Graduate School of Arts and Sciences, founded in 1872), Harvard Athletics and the Division of Continuing Education. The 40 academic departments and 30+ centers of the FAS support a community unparalleled in its academic excellence across the broadest range of liberal arts and sciences disciplines. Together, the FAS seeks to foster an environment of ambition, curiosity and shared commitment to knowledge and truth that elicits excellence from all members of our community and prepares the next generation of leaders through a transformative educational experience.

Job Description

Job Summary

The Wagers laboratory studies the biology and function of adult tissue-specific stem cells, particularly those stem cells that form the skeletal muscle and blood.  Current topics under investigation include regulation of stem cell function in normal tissues and under abnormal conditions such as aging and cancer, and methods for editing stem cell genomes using CRISPR/Cas9 for experimental and therapeutic purposes.  The lab is seeking highly motivated candidates to assist in ongoing experiments that include the development and application of novel CRISPR/Cas9 and adeno-associated virus (AAV) gene transfer technologies, mouse work (colony maintenance, husbandry, genotyping, physiological assessment, tissue harvest, etc.), cell culture, flow cytometry, immunostaining, and bioassays of skeletal muscle and hematopoietic cell function. The assistant will also perform a variety of basic and general lab support and clerical tasks, including ordering and stocking of supplies, maintenance of equipment and preparation and storage of shared reagents. They will also be expected to participate in and present results for regular research group meetings and to contribute to the preparation and presentation of written and oral progress reports, as needed. Work will be performed under the specific direction of the PI, research manager and/or senior lab members, although certain research-related tasks will be performed independently, within the guidelines of and subject to review by the PI and research staff.

Job-Specific Responsibilities

Performs laboratory experiments utilizing AAVs and CRISPR/Cas9 genome editing complexes, gene engineered mouse models, cell culture, flow cytometry, immunostaining, microscopy, molecular biology and bioassays;

Collects and safeguards project data; keeps detailed, accurate and up-to-date notes detailing experimental design and results;

Under direction, processes, organizes and summarizes data, reporting experiment results in written form and in oral presentations, using a variety of scientific, word processing, spreadsheet and statistical software applications or program platforms;

As a member of a project team, assists in the design and execution of laboratory experiments, techniques, and protocols;

Performs routine logging and/or testing of samples; routine animal husbandry and experimentation, and genotyping

May occasionally instruct others in basic laboratory techniques or procedures;

Performs related laboratory maintenance such as maintaining and cleaning equipment, scheduling repairs, tracking inventory and ordering supplies.

Working Conditions and Physical Requirements

May be required to lift, move and transport related laboratory equipment.

Required to work with a variety of hazardous materials under approved safety protocols.

Required to work with and handle animals.

This position is based in Cambridge, MA

Qualifications

Basic Qualifications

At least one year related work experience (relevant course work may count towards experience)

Additional Qualifications and Skills

Previous undergraduate or post-graduate lab experience, particularly with gene editing systems, animal models and tissue histology will be preferred. Demonstrated abilities in relevant laboratory techniques is also preferred. Exposure to applicable computer technologies, including specific software applications, is encouraged. Appropriate organizational and communications skills required.

Additional Information

Appointment End Date: This is a one year grant funded term position with the possibility of extension based on availability of continued funding and satisfactory performance.

Standard Hours/Schedule: 35 hours per week

Visa Sponsorship Information: Harvard University is unable to provide visa sponsorship for this position

Pre-Employment Screening: Identity

Other Information

All formal offers will be made by FAS Human Resources

Work Format Details

This position has been determined by school or unit leaders that all duties and responsibilities must be performed at a Harvard or Harvard-designated location. Certain visa types may limit work location. Individuals must meet work location sponsorship requirements prior to employment.

Salary Grade and Ranges

This position is salary grade level 052. Please visit  Harvard's Salary Ranges  to view the corresponding salary range and related information.

Benefits

Harvard offers a comprehensive benefits package that is designed to support a healthy work-life balance and your physical, mental and financial wellbeing. Because here, you are what matters. Our benefits include, but are not limited